Abstract: Objective To investigate the association between IVS10+12G＞A and IVS12-6T＞C polymorphisms in the hMSH2 gene and incidence of familial or under 50 age gastric cancer.
MethodsA casecontrol study has been taken on subjects including 149 sporadic cases， 100 familial cases and 260 healthy individual controls to investigate whether such polymorphisms affecting the risk of developing gastric cancer. Peripheral blood cell DNA was obtained from all subjects. IVS10+12 G＞A and IVS12-6T＞C polymorphisms in hMSH2 gene were detected using a PCRbased DHPLC and verified by DNA sequencing.
ResultsThe frequencies of IVS10+12 G＞A and IVS12-6T＞C polymorphisms in 249 patients were significantly higher than those in 260 controls. IVS10+12 G＞A and IVS12-6T＞C polymorphisms jointly affected the onset of gastric cancer，especially in familial cases. No difference was found between 149 sporadic patients and 260 controls. The stratification analysis of confounding factors showed that hMSH2 gene IVS10+12 G＞A and IVS12-6 T＞C polymorphisms exist mainly in under 50 age patients（P＜0.05）， and significantly correlated with sauerkraut dietary factor（P＜0.05）.
ConclusionThe IVS10+12G＞A and IVS12-6T＞C polymorphisms in hMSH2 gene are associated with increased risk of developing gastric cancer in familial or under 50 age population. Determination of these polymorphisms may be suitable to identify individuals with increased risk of gastric cancer.