Abstract:

Objective To detect the expression of MACC-1 mRNA and protein in colon carcinoma and investigate the relationship with clinicopathologic characteristics. Methods The expressions of MACC-1 in 48 colon caner tissues and corresponding adjacent normal tissues were detected by realtime quantitative reverse transcription polymerase chain reaction（QT-PCR） and Western blotting. Results The expression level of MACC-1 mRNA and protein in colon cancer tissues was higher than those in adjacent normal tissues. The relative quantitative expression of MACC-1 mRNA in colon cancer tissues was （8.52 ± 2.39）×10-4， which significantly higher than （1.47 ±1.24）×10-5 of adjacent normal tissues（P＜0.05）. The expression of MACC-1 protein in colon cancer tissues was 7.73±0.03, which higher than 1.02±0.01 of adjacent normal tissues（P＜0.05）. The expression of MACC-1 mRNA and protein were also positively correlated with histologic types,infiltration depth and TNM stage （P＜0.05）.No correlation was found in age or sex （P＞0.05）. Conclusion MACC-1 may promote the growth and metastasis of colon cancer. It will be a prognostic markers for colon cancer.