Abstract: Objective To investigate the expression of D2-40 and CD34 with lymphatic vessel density（LVD） marked by D2-40 and microvessel density（MVD） marked by CD34 in gastric cancer and the correlation of them with clinical pathological features. Methods Expressions of D2-40 and CD34 were detected in 108 cases of gastric cancer and 36 cases of adjacent normal tissues using immunohistochemistry and tissue microarray. The LVD marked by positive D2-40 and MVD by positive CD34 were calculated，and the correlation with the clinical pathological features and prognosis were analyzed. Results The positive rate of D2-40 in gastric cancer was 85.2％（92／108），higher than 22.2％（8／36）in adjacent normal tissue with significance（P=0.000）；the positive rate of CD34 was 94.4％（102／108）in gastric cancer，higher than 36.1％（13／36） in adjacent normal tissue with significance（P=0.000）.The LVD（10.14±5.37）and MVD（45.32±15.78）in gastric cancer were significantly higher than 3.38±1.69 and 4.92±1.26 in adjacent normal tissues（P=0.000，P=0.000）. The expression of D2-40 LVD and CD34MVD in the malignancy were related to the tumor size，invasion depth，lymphatic metastasis and TNM stage，but not with age and sex. Cox multivariant analysis showed that the tumor size, invasion depth, lymphatic metastasis, TNM stage, MVD and LVD were independent factors affecting prognosis. The Kaplan-Meier analysis showed that the median overall survival of the fewlymphatic vessel group was 69.0 months（95％ CI：59.91-77.20 months） and that of the multi-lymphatic vessel group was 33.0 months（95％ CI：23.20-42.66months） with significance；the median overall survival of few-blood microvessel group was 67.0 months（95％ CI：58.10-76.39 months）and that of multi-blood microvessel group was 34.0 months（95％ CI：24.63-44.02 months） with significance（P＜0.05）. Conclusion The LVD marked by D2-40 and MVD marked by CD34 are positively correlated to the clinicopathological factors of the malignancy， and may play an predictive role in the development and progression of gastric cancer.