Abstract: Objective To explore the expression of focal adhesion kinase（FAK） in different migration characteristics of osteosarcoma subclone cell lines，as well as its role in the osteosarcoma cell proliferation and migration. Methods Limiting dilution was used to obtain 143B subclone cell lines（A1，A2，A3，A4，A5）with different migration capacities，and their FAK expressions were detected by Western blottting. The role of FAK in the osteosarcoma cells proliferation and migration was studied by small interfering RNA（siRNA） sequence，and the proportion of the cells with lamellipodia was counted by immunofluorescence staining method.
ResultsThe migration ability of osteosarcoma cell lines 143B subclone A2 and A3 was significantly stronger than A1, A4 and A5（P＜0.05），and FAK expressions of cell lines A2 and A3 were significantly higher. The subclone cell line A3 transfected with FAKsiRNA decreased the migration ability（P＜0.05），and the proportion of cells with lamellipodia decreased from 33.3％ to 12.8％（P＜0.05），but there was no change of the cell growth. Conclusion FAK was founded in the 143B osteosarcoma subclone cell lines with stronger migration capacities. FAK may affect the migration ability of osteosarcoma cells through lamellipodia formation，but have no effect on the proliferation of osteosarcoma cells.