Abstract: Objective To investigate the expression and clinical significance of anexelekto（Axl） and its ligand growth arrest specific 6（Gas6）in breast cancer. Methods Axl and its ligand Gas6 expression were examined in 96 cases of breast cancer and paired adjacent normal tissues by immunohistochemistry. The association between Axl and Gas6 protein expression and clinicopathological characteristics in breast cancer was further analyzed. Results Axl expression in breast cancer tissue（62.5％, 60/96） was higher than that in adjacent normal breast tissue（17.7％, 17/96） with significance（P＜0.05）. Axl ligand Gas6 expression in breast cancer tissue（54.2％， 52/96） was higher than that in adjacent normal breast tissue（12.5％, 12/96） with significance（P＜0.05）. There was no relationship between Axl expression and age，tumor size，histological grade and c-erb-B2 expression, but it correlated with lymph node metastasis, ER and PR espression. Gas6 expression was correlated with lymph node metastasis, histological grade and ER expression, but not with age, tumor size, PR and c-erb-B2 expression. Axl expression was positively correlated with Gas6 expression in breast cancer（r=0.540，P＜0.001）. Conclusion Axl and Gas6 play an important role in the occurrence and invasion in breast cancer.