Abstract: Objective To investigate the expression of survivin in osteosarcoma MG-63 cells and the effect of survivin antisense oligonucleotide（ASODN）on its proliferation and apoptosis. Methods The ASODN and sense oligonucleotide（SODN）targeting survivin were designed and constructed. The liposome／oligonucleotide complexes were prepared by adding oligonucleotide and then transferred into MG-63 cells. According to the protocol，the MG-63 cells were assigned into Lip group（empty liposome），Lip-SODN group（liposomemediated SODN）and Lip-ASODN group（liposome-mediated ASODN）. The immunohistochemical staining was used to detect the expression of survivin in MG-63 cells. Western blotting assay was employed to measure the protein levels of survivin at 48th hour after transfection of each group. The proliferation at 24th，48th and 72nd hour and apoptosis at 48th hour after transfection were measured by MTT and flow cytometry，respectively. Results The survivin was mainly expressed in the nucleus of MG-63 cells. The protein level of Lip-ASODN group was lower than that of Lip group and Lip-SODN group. The Lip-ASODN group's proliferation inhibition rate was superior to Lip group in a dose-dependent manner, while the difference between Lip-SODN group and Lip group was not significant（P＞0.05）. The apoptotic rate of Lip-ASODN group was（88.47±0.79）％，higher than（10.01±0.90）％ of Lip group and（4.12±0.25）％ of Lip-SODN group with significant difference（P＜0.05）.
Conclusion The ASODN targeting survivin can inhibit the proliferative capacity and promote the apoptosis of osteosarcoma MG-63 cells.