Abstract: Objective To detect the expression of macrophage migration inhibitory factor（MIF）， interleukin（IL）-17 and IL-10 in hepatocellular carcinoma（HCC）， and discuss their clinical significance.
Methods Fifty-two cases of HCC tissues， the adjacent liver cirrhosis（LC） tissues of HCC and 20 cases normal liver samples in the department of hepatobiliary surgery, Lanzhou General Hospital, Lanzhou Command, from September 2010 to March 2013 were collected. Immunohistochemistry technique was used to detect the expression of MIF， IL-17 and IL-10 in tissues of three groups. The relationship between the expression and clinicopathological features of HCC， such as the tumor size， degrees of differentiation and other features were analyzed.
Results The positive rates of MIF， IL-17and IL-10 were 78.8％，76.9％，28.8％ in HCC tissues respectively；75.0％，73.0％，32.7％ in LC tissues；and 20.0％，25.0％，70.0％ in the normal liver tissues. Compared with the normal liver tissue， the MIF and IL-17 expression of HCC and LC tissues were higher， and IL-10 expression was lower， with statistical significances （P＜0.05）. The expression of MIF， IL-17 and IL-10 in HCC tissues was related to the tumor size， degrees of differentiation， not related to age， gender， tumor thrombus and ChildPugh stage. The different expression of MIF, IL-17, IL-10 in HCC tissues was related to one year survival rate of HCC patients.
Conclusion The expression of MIF， IL-17 and IL-10 are closely correlated with the occurence, development and prognosis of HCC.