Abstract: Objective To investigate the expression profiles of mRNA between non-small cell lung cancer（NSCLC） tissues and normal lung tissues， and analyze bioinformatics information. Methods Gene microarray was used to explore mRNA expression profile in 3 cases of NSCLC tissues（NSCLC group） and 3 normal lung tissues（normal group）. The differentially expressed genes were selected and Gene Ontoloty（GO） analysis was performed as well as path way analysis. Results Among 20 716 genes， 896 differentially expressed mRNAs were found， 593 mRNAs were upregulated and 303 mRNAs were downregulated in the NSCLC group compared with the normal group， respectively. Bioinformatics analysis revealed that differential expression mRNA involved in significant biological regulatory function and pathways. Conclusion Genome microarray can detect differentially expressed genes in NSCLC tissues and normal lung tissues. Patterns of gene expression in the NSCLC tissues and normal lung tissues are significant different. The differential expression mRNAs were crucial in significant biological function.