Abstract: Objective To explore the effect of up-regulation of microRNA-7（miR-7） on proliferation and metastasis of Hep-2 laryngeal carcinoma cells and its possible mechanism. Methods The Hep-2 laryngeal carcinoma cells were divided into miR-7 group（Hep-2 cells transfected with miR-7 mimics）， Scramble group（Hep-2 cells transfected with miR-Scramble） and Mock group（Hep-2 cells without transfection）. The quantitive real-time PCR（qPCR） was used to test the expression of miR-7 at 48 h after transfection in 3 groups. The CCK-8 assay was used to test the proliferation of Hep-2 cells in each group at 48 h after transfection. Cell metastasis was determined by transwell test at 48 h after transfection. The mRNA and protein levels of PI3K（p110）， AKT and pAKT were examined by qPCR and Western blotting. Results The expression of miR-7 was significantly up-regulated after transfection with miR-7 mimics. The proliferation and metastasis of Hep-2 laryngeal carcinoma cells was extremely decreased after transfection with miR-7. The results from qPCR and Western blotting showed that the mRNA and protein levels of PI3K（p110）， AKT and pAKT were decreased in miR-7 group compared with other two groups（P＜0.05）. Conclusion Up-regulation of miR-7 can inhibit the proliferation and metastasis ability of Hep-2 laryngeal carcinoma cells with possible mechanism of inhibiting PI3K／AKT signal pathway.