Abstract: Objective To investigate the expression of regenerating gene Ⅳ（RegⅣ） in human endometrial carcinoma，atypical hyperplasia of endometrium and normal endometrium tissues， and analyze its correlation with clinicopathlogical characteristics and prognosis in endometrial carcinoma. Methods Immunohistochemistry method was used to detect the expression of RegⅣ in 138 cases of endometrial carcinoma，25 cases of atypical hyperplasia of endometrium and 31cases of normal endometrium. Then the relationship between the expression of RegⅣ and clinicopathlogical characteristies of endometrial carcinoma was analyzed. The overall survival of patients was estimated using the Kaplan-Meier method. The factors affecting the prognosis of endometrial carcinoma was analyzed by Cox multivariate survival analysis. ResultsThe positive expression rates of RegⅣ in normal endometrium，atypical hyperplasia of endometrium and endometrial carcinoma tissues were 9.7％（3／31）， 32.0％（8／25） and 60.1％（83／138）， respectively. The differences among the three groups was statistically significant（P＜0.05）. The expression of RegⅣ was correlated with the age of endometrial carcinoma patient， surgical pathologic stage，grade of histological, invasive depth and lymph node metastasis（P＜0.05）， but no significant difference among the pathological types（P＞0.05）. Cox multivariate survival analysis showed that gene expression of RegⅣ was the independent predictor from disease free survival of patients with endometrial cancer. Conclusion RegⅣ may contribute to the development of endometrial carcinoma， and guide the evaluation for prognosis.