Abstract: Objective To investigate the expression and clinical significance of AKR1C1 in non-small cell lung cancer（NSCLC） tissues and cell line. Methods Immunohistochemical SP method was used to examine the expression of AKR1C1 in 50 cases of NSCLC tissues and matched adjacent normal tissues. NCI-H460 cell was transfected with AKR1C1 siRNA（AKR1C1 interference group） and AKR1C1 negative control siRNA（negative control group）. Western blotting was used to confirm the results of transfection. The proliferation， colony formation and invasion ability were analyzed by MTT， low-density cell colony formation， woundinghealing and transwell experiments. The blank control group was set. Results The high-level expression rates of AKR1C1 in cancer tissues were 94.0％（47／50）， which was higher than 6.0％（3／50）in matched adjacent normal tissues （P＜0.05）. MTT analysis showed the proliferation rate of AKR1C1 interference group and negative control group was the same. The colony formation number of AKR1C1 interference group， negative control group and blank control group were 69.60±4.03， 69.00±1.63 and 70.33±2.05， with no significant difference. The number of migrated cells in AKR1C1 interference group was 15.30±2.50， which was lower than that of negative control group （30.00±2.20） and blank control group （31.30±2.40），with significant differences（P＜0.05）. The relative migrated distance of the cells in AKR1C1 interference group was 0.13±0.05， which was lower than that negative control group （0.60±0.08）and blank control group（0.56±0.05），with significant differences（P＜0.05）. Conclusion AKR1C1 is high expressed and may relate to the migration in NSCLC.