Abstract: Objective To investigate the clinical significance of serum levels of lactate dehydrogenase（LDH） and β2-microglobulin（β2-MG） in diffuse large Bcell lymphoma（DLBCL）. Methods The serum levels of LDH and β2-MG of 58 patients with DLBCL were measured by rate method and radioimmunoassay. Besides， the clinicopathological characteristics of the patients such as gender， age， B symptoms， clinical stages， ECOG score of performance status（ECOG-PS） and bone marrow invasion were summarized and anylyzed. Meanwhile， the association between the level change of LDH and β2-MG after chemotherapy and therapeutic effectiveness was also evaluated. Results The levels of LDH and β2MG in the advanced stage group，ECOG≥2 group， bone marrow invasion group were significantly higher than those of their counterpart （P＜0.05）.The level of β2MG in aged over 60 patients was higher than that of younger ones （P＜0.05），but there was no statistical significance in the LDH level（P＞0.05）. The level of LDH in B symptoms group was higher than that of without B symptoms（P＜0.05），but there was no statistical significance in the β2MG level（P＞0.05）. There was no statistical significance about LDH and β2MG levels in gender（P＞0.05）. The levels of LDH and β2-MG in positive response group significantly decreased after chemotherapy （P＜0.05）. Conclusion LDH and β2-MG can be taken as indicators in classifying the clinical phase and judging whether there is bone marrow infiltration or not， and evaluating the chemotherapeutic effectiveness of DLBCL patients.