Abstract: ObjectiveTo investigate the effect of morphine（Mor）combined with cisplatin（DDP）on the proliferation，apoptosis, as well as invasion and metastasis of human ovarian cancer Skov3 cells. Methods MTT method was used to observe the cell survival rates at 24，48，72，96 h after the treatment of mor at different concentrations（10，20，50，100，200 μmol／L）and DDP（1，2，5，10 and 20 mg／L） as to screen the concentration of Mor and DDP for subsequent functional study. Skov3 cells at logarithmic growth phase were divided into 4 groups：Mor（200 μmol/L）group，DDP（20 mg/L）group，Mor（200 μmol/L）+DDP（20 mg/L） group and control group（Skov3 cells without treatment）. Flow cytometry and real-time quantitative PCR were used to detect the apoptosis rate and mRNA levels of apoptosis related genes（Bcl-2，caspase-3 and Bax）at 48 h after the treatment. Transwell test and Western blotting were used to detect the number of cells and the invasion associated proteins（TIMP-2，TIMP-1 MMP-9 and MMP-2）at 48 h after treatment. Results The proliferation inhibition rates increased gradually with the increase of Mor（10-200 μmol／L）and DDP（1-20 mg／L）concentration. The inhibitory effect was in a time and concentration dependent manner. The proliferation inhibition rates of Mor+DDP group was higher than those of Mor group，DDP group and control group at 24, 48，72，96 h after treatment（P＜0.05）. Compared with control group，there were increased apoptosis rate，caspase-3 activation rate and mRNA levels of Bax and caspase-3, as well as protein levels of TIMP-1 and TIMP-2，but decreased Bcl-2 mRNA，number of cells penetrating membrane and protein levels of MMP-9 and MMP-2 in cells treated with Mor or DDP alone or in combination（P＜0.05）. The levels of the above indexes in Mor+DDP group were better than those in Mor or DDP groups（P＜0.05）. Conclusion Both Mor and DDP can inhibit the proliferation of Skov3 cells and induce apoptosis，and the inhibition of Mor combined with DDP is enhanced.