Abstract: Objective To examine the expressions of special AT-rich sequence binding protein-1（SATB1），kisspeptin-1（KISS1）in human gastric adenocarcinoma and investigate their relevance and clinical significance. Methods The expressions of SATB1 and KISS1 were detected in 76 gastric adenocarcinoma and 19 adjacent normal gastric tissues by tissue chip technology and immunohistochemical staining. The correlation of the expressions with clinicalpathological features and prognosis were analyzed.Results The positive rate of SATB1 and KISS1 in gastric adenocarcinoma tissues were 80.3％（61／76） and 14.5％（11／76）respectively. Compared with 10.5％（2／19） and 89.5％（17／19）of normal gastric tissue，the differences were statistical significantly（P＜0.05）. The SATB1，KISS1 expressions were related to invasive depth， metastasis and TNM staging（P＜0.05），but not related to age，sex，tumor diameter and histological differentiation（P＞0.05）.SATB1 was negatively correlated with KISS1（r=-0.736，P＜0.01）. The median overall survival（OS）of SATB1 positive and negative patients was 16.8 months（95％CI：10.124-21.876 months）and 42.2 months（95％CI：31.804-52.196 months），and the difference was statistically significant（P＜0.05）. The median OS of KISS1 positive and negative patients was 45.1 months （95％ CI：36.736-53.264 months）and 13.6 months（95％ CI：9.971-16.029 months），and the difference was statistically significant（P＜0.05）. Conclusion SATB1 and KISS1 play important roles in carcinogenesis and progression of gastric adenocarcinoma， which can be regarded as useful biomarkers to evaluate the biological characteristics of gastric adenocarcinoma.