Abstract:

bjective To explore the effects of morphine on the proliferation and apoptosis of A549 cells. Methods The CCK-8 was used to detect the

proliferation of A549 cells at 48 h after treatment with different concentrations of morphine （0.3，3，30 μg/ml）. Annexin-V FITC/PI double staining was used to detect the apoptosis rate of 30 μg/ml morphine at 48 h. Western blotting was used to detect the expression levels of X-linked apoptosis inhibitory protein （XIAP）， survivin， Bcl-2， caspase-3 and Bax of 30 μg/ml morphine at 48 h. Results Morphine could dose-dependently stimulate the proliferation of A549 cells at different concentrations， and the differences were statistically significant （P＜0.05）. Compared with the control group， the apoptotic rate of A549 cells was decreased after treatment with 30 μg/ml morphine （P＜0.05）. The treatment with morphine leaded to the increased levels of Survivin and XIAP but decreased level of caspase-3（P＜0.05）. Conclusion Morphine could promote proliferation and inhibit the apoptosis of A549 cells，possibly via the upregulation of XIAP and survivin and inhibition of caspase-3 activation.