Abstract: Objective To explore the expression of fibroblast growth factorinducible 14（Fn14）and Janus kinase／signal transducerand activator of transcription（JAK／STAT）in non-small cell lung cancer（NSCLC）tissues with EGFR exon 19 deletion. Methods In this study，125 NSCLC cases with EGFR 19-Del were selected by amplification refractory mutation system. And the 30 cases of paired adjacent normal tissues were collected. Expressions of Fn14，p-JAK1 and p-STAT1 were detected in these samples using immunohistochemistry and the correlation of Fn14 expression with JAK／STAT expression was analyzed by statistics. Results The expression of Fn14 was mainly located on cell membrane and cytoplasm, while the expression of p-JAK1 and p-STAT1 were mainly located in cytoplasm and cell nucleus. Compared to normal lung tissues，the higher expression rates of Fn14，p-JAK1 and p-STAT1 protein were 100.0％（125／125），83.2％（104／125）and 100.0％（125／125）in NSCLC tissues with EGFR 19-Del（P＜0.05）. There were significant differences of Fn14，p-JAK1 and p-STAT1 expression in histological grades，pTNM stages and lymph node metastasis（P＜0.05），while there were no significant differences in sex，age，smoking history and pathological types（P＞0.05）. The correlation of Fn14 with p-JAK1 and p-STAT1 were obvious（P＜0.05，r＞0.3）in pathological classification，histological grads and pTNM stage of NSCLC. Conclusion Fn14，p-JAK1 and p-STAT1 proteins are highly expressed in NSCLC with EGFR 19-Del，suggesting that the above three proteins would be likely to associate with the development of NSCLC. The good correlations between Fn14 expression and p-JAK1，p-STAT1 hints that Fn14 may effect on the expression of p-JAK1，p-STAT1. All these provide important basis for the mechanism study of Fn14 affecting on the development of NSCLC with EGFR 19-Del.