Abstract: Objective To explore the expression of long non-coding RNA of colon cancer associated transcript 1（LncRNA-CCAT1）in hepatocellular carcinoma（HCC）tissues and cell lines, as well as its clinical significance. Methods CCAT1 expression in 42 pairs of HCC tissues and paired adjacent normal tissues as well as 4 types of HCC cell lines（SMMC-7721，Hep3B，Hub7 and HepG2）and normal hepatic cell line LO2 were tested by QPCR. The relationship between the expression of CCAT1 and common clinical pathological parameters（gender，age，alpha-fetoprotein，tumor size，tumor number，lymph node metastasis，differentiation and TNM stage）were analyzed accordingly. Results Compared with normal hepatic cell line LO2，there was a high expression of CCAT1 in SMMC-7721, Hep3B, Hub7 and HepG2 cell lines with significant difference（P＜0.05）, especially in HepG2 cell line. The expression of CCAT1 in HCC tissue was higher than that in the adjacent tissues，and the difference was statistically significant（P＜0.05）. The expression of CCAT1 in HCC was related with tumor size，alpha fetoprotein，differentiation，TNM stage and lymph node metastasis（P＜0.05）, but not with age, gender and tumor number（P＞0.05）. Conclusion LncRNA CCAT1 has a role in the occurence,development and prognosis of HCC，worthy of further research on its mechanism.