Abstract: Objective To explore the expression and clinical significance of Kif2c in non-small cell lung cancer （NSCLC）. Methods Specimens containing 62 surgically resected NSCLC tissues and 30 paracancerous tissues were selected from the First Affiliated Hospital of Bengbu Medical College. Immunohistochemical staining was applied to detect the expression of Kif2c in NSCLC tissues and paracancerous tissues. The relationship between clinical characteristics and Kif2c expression was also studied. Results The high-expression rate of Kif2c in NSCLC tissues was 75.8% （47/62）， higher than 26.7% （8/30） of paracancerous tissues with significant difference （P<0.05）. The expression of Kif2c protein was closely related to the clinical stage and lymph node metastasis of NSCLC. The high-expression rate of Kif2c in clinical stage Ⅲ was significant higher than those of stage Ⅰ-Ⅱ in NSCLC tissues （P<0.05）. The high-expression rate of Kif2c in lymph node metastasis was significant higher than those without lymphnode metastasis （P<0.05）. The high-expression rates of Kif2c protein in NSCLC had no relationship with sex，age， tumor size，grade and organization type（P>0.05）. Conclusion Kif2c may play an important role in the occurrence and development of NSCLC. Kif2c can be a molecular target of NSCLC.