Abstract: Objective To investigate the effect of naringin on the proliferative inhibition of cisplatin （DDP）resistant ovarian cancer SKOV3／DDP cells in vitro and its reverse effect of drug resistance， as well as the possible mechanisms. MethodsDDP to half maximal inhibitory concentration （IC₅₀） on SKOV3 and SKOV3／DDP cells was determined by MTT method. The proliferatiive inhibition of naringin alone and naringin in combination with DDP on SKOV3／DDP cells was tested by MTT method. Non-cytotoxic naringin was selected， and SKOV3／DDP cells were divided into blank control group， 2.5 μg／ml DDP group， 10 μmol/L naringin group and 10 μmol/L naringin+2.5 μg／ml DDP group. RTPCR and Western blotting was used to detect the MDR1 and MRP2 mRNA levels and protein expressions of Pgp and MRP2. ResultsAfter treated with 1.32 μg／ml DDP for 48 h， the IC₅₀ of SKOV3 cells and SKOV3／DDP cells were 5.48 μg／ml and 14.93 μg／ml， and the resistance index （RI） was 2. 72. Naringin could effectively restrain the proliferation of SKOV3／DDP cells in a time and dose dependent manner. The dose of 10 μmol／L naringin was chosen for the further experiment. The RI was 1.62 and the drug resistance reversal fold was 1.68 times after 10 μmol／L naringin+2.5 μg／ml DDP acted on SKOV3／DDP cells. The mRNA levels of MDR1 and MRP2， as well as the protein expressions of P-gp and MRP2 decreased significantly in 10 μmol／L naringin+2.5 μg／ml DDP group compared with 2.5 μg／ml DDP group （P＜0.05）. ConclusionNaringin can significantly inhibit the proliferation of SKOV3／DDP cells in vitro， and can enhance the sensitivity of SKOV3／DDP cell to DDP. The reversal mechanism of drug resistance may be related to the inhibition of MDR1 and MRP2 mRNA and related proteins.