Abstract: Objective To explore the effect of ginkgolide B（GB）on the proliferation， apoptosis， migration and invasion of osteosarcoma（OS）cells as well as the potential mechanisms. Methods Human OS Saos-2 cells were incubated with 300，600 and 1200 μmol/L GB for 24， 48 and 72 h. We detected cells viability，apoptosis， migration and invasion with cell counting kit-8（CCK-8）， flow cytometry and Transwell assay，separately. Using the extraction of the mRNA and protein of Saos-2 cells pretreated with GB，we detected the mRNA and protein levels of Bcl-2，Bax，caspase-3，MMP-2 and MMP-9 by using quantitative real-time PCR and Western blotting. The phosphorylation of Erk and Akt and level of cleaved-caspase-3 were detected by Western blotting. Results GB significantly inhibited the proliferation of Saos-2 cells in a time-and dose-dependent manner. GB at 600 and 1200 μmol/L can induce the apoptosis of OS cells. GB exerted a dose-dependent role in the suppression of migration and invasion of OS cells. GB could increase the mRNA and protein levels of Bax，but decreased the mRNA and protein levels of Bcl-2， MMP-2 and MMP-9. The high concentration of GB could inhibit the phosphorylation of Erk and Akt and increase the level of cleaved-caspase-3. Conclusion GB could inhibit the proliferation， migration and invasion of Saos-2 cells and induce apoptosis，which may be related to the inhibition of Erk and Akt phosphorylation.