Abstract: Objective To investigate the expression of epsteinbarr virus latent membrane protein 2A（LMP2A） in gastric cancer tissues and its correlation with clinicopathological parameters and prognosis. Methods LMP2A expression in 100 cases of gastric cancer tissues and paracancerous tissues was detected using immunohistochemistry. The correlation of LMP2A with clinical parameters was analyzed. The survival curves were drawn using KaplanMeier method and the correlation of LMP2A with overall survival（OS） and progression free survival（PFS） was analyzed in followed-up 76 patients. Results There was nearly no expression of LMP2A in paracancerous tissue. High expression of LMP2A was detected in gastric cancer tissues and the high expression rate was 47.0％（47／100）. High LMP2A expression was positively correlated with lmphytic invasion， vessel invasion and advanced clinical stage（P＜0.05）. The median OS and PFS of high LMP2A expression group was 52.0（95％CI：43.8-60.2）months and 47.0 months（95％CI：32.9-61.1）, respectively. The median OS and PFS of low LMP2A expression group were not reached. There was significantly statistical difference between both groups（P＜0.05）. Conclusion LMP2A was high expressed in gastric cancer. It may play an important role in the development of gastric cancer.