Abstract: Objective To explore the expression of sex determining region Y-box 2（SOX2）in gastric cancer and its realationship with clinicopathological characteristics and provide reference for clinic. Methods Databases including PubMed， EMBASE， CNKI， Wanfang and VIP were searched from inception to October 1, 2016 for studies about the relationship between expression of SOX2 and the clinicopathological characteristics of gastric cancer. The data were screened and extracted according to the inclusion and exclusion criteria. The Cochrane Collaborations software RevMan 53 was used to test the heterogeneity， overall effect and publication bias of the combined studies. Results A total of 13 studies including 1492 cases of gastric cancer tissues and 724 cases of normal gastric tissues were recruited. The results of the meta-analysis showed that as for the positive rate of SOX2 expression， significant differences were tested between gastric cancer tissues vs. normal gastric tissues， well and moderate differentiation vs. poor differentiation with OR（95％CI） at 0.16（0.11-0.23） and 2.66（1.54-4.59） respectively. No significant difference was tested between lymph node metastasis vs. nonlymph node metastasis， clinical stages Ⅰ+Ⅱ vs. clinical stages Ⅲ+Ⅳ， the infiltration depth of T1+T2 vs. T3+T4, male vs. female with OR（95％CI） at 0.54（0.26-1.11）， 2.23（0.82-6.09）, 1.44（0.64-3.22） and 1.10（0.82-1.47）, respectively. Conclusion SOX2 expression might be associated with gastric cancer. However， more highquality studies are expected for further studies.