Abstract: At present， more and more research indicates that Celltracks® AutoPrep® misses circulating tumor cells （CTCs） with atypicalepithelial characteristics，which have a stronger ability of invasion and metastasis than CTCs simply expressing epithelial characteristic and a higher value to judge efficacy and prognosis. This paper reviews the contrast between Celltracks® AutoPrep® system and ISET technology， and the effect of EpCAM expression， hybrid， EMT+tumor， circulating tumor stem cells and irreversible EMT+tumor cells on the detection rate of Celltracks® AutoPrep® system， as well as the feasibility of Celltracks® AutoPrep® system for detecting CTCs.