Abstract: Objective To investigate the feasibility of right upper lobectomy with single utility port video-assisted thoracoscopic surgery（VATS）for lung cancer. Methods The clinical data of 25 patients who underwent right upper lobectomy with single utility port VATS from December 2015 to December 2016 were retrospectively analyzed. The operation time，blood loss，extubation time and hospitalization days were observed. Results The procedure was successfully performed in all patients and no patient transferred for open thoracotomy. The mean time of operation was（90±10.6）min，and the mean blood loss was（60±5）ml. The mean extubation time was（5±0.5）d，and the mean hospitalization days was（9.7±0.5）d. Four patients were suffered from postoperative atelectasis with lung infections. Conclusion The right upper lobectomy with single utility port VATS for lung cancer of right upper lobe is simple，safe and reliable，but it requires good differentiation of lung and surgical skill，and worthy of wide application