关键词: 小细胞肺癌')">

小细胞肺癌, 生物信息学, 竞争性内源RNA, 长链非编码RNA

Abstract:

【Abstract】Objective  To screen ncRNAs involved in the development of small cell lung cancer（SCLC） and predicted potential targets for its diagnosis and treatment. Methods Differential gene analysis was performed on the expression profile microarray data obtained from Gene Expression Omnibus (GEO), ceRNA network was constructed, and functional enrichment analysis and PPI network construction were performed. A total of 7 patients with surgically treated small cell lung cancer were collected, and some differential genes (lncRNA, mRNA and miRNA) were validated in clinical samples using qPCR. Results A total of 99 lncRNAs, 46 miRNAs and 73 mRNA molecules were involved in the construction of the regulatory network. GO and KEGG analyses indicated that the dysregulated mRNAs were mainly involved in cellular cancerization, proliferation and metastasis in small cell lung cancer. According to the PPI network analysis, 46 genes were considered hub genes. qPCR Results  showed that compared to adjacent normal tissues, lnc-ENST00000430027.3 showed relatively high expression (P＜0.001), hsa-miR-143-5p showed relatively low expression (P＜0.01) and FANCA showed relatively high expression (P＜0.01) in small cell lung cancer tissues. Conclusion Combined with the validation of clinical specimens for some genes, it provides a new basis for the molecular biology study of small cell lung cancer diagnosis and treatment.

Key words: Small cell lung cancer(SCLC), Bioinformatics analysis, ceRNA, lncRNA