关键词: 老年喉鳞状细胞癌, PAC-1, CD62P, SLC7A11, 预后, 危险因素

Abstract:

【Abstract】Objective To analyze the expression levels of platelet membrane glycoprotein Ⅱb/Ⅲa fibrinogen receptor (PAC-1), platelet P-selectin (CD62P) and SLC7A11 on platelet surface and their clinical significance in elderly patients with laryngeal squamous cell carcinoma. Methods Eighty-seven elderly patients with laryngeal squamous cell carcinoma admitted to our hospital from January 2016 to January 2021 (laryngeal cancer group) were collected, and 87 patients with vocal cord polyp admitted to our hospital during the same period (control group) were selected. Blood and pathological specimens were collected from the patients, and the positive rate of specific fluorescent antibody binding platelets was determined by flow cytometry using monoclonal immunofluorescence labeling, PAC-1 and CD62P were detected, and expression of SLC7A11 was detected by immunohistochemical SP method. The relationship between the expressions of PAC-1, CD62P and SLC7A11 and the prognosis of patients was analyzed. Results The positive expression rate of PAC-1 and CD62P in laryngeal cancer group was higher than that in control group, and the high expression rate of SLC7A11 (62.07%) was lower than that in control group (28.74%), the difference was statistically significant (P＜0.05). Among the 87 patients with good prognosis, 69 patients had good prognosis, and 18 patients had poor prognosis. The positive expression rate of PAC-1 and CD62P in the poor prognosis group was higher than that in the good prognosis group, and the high expression rate of SLC7A11 (94.44%) was higher than that in the good prognosis group (53.62%) (P＜0.05). The proportion of clinical stage and cervical lymph node metastasis in patients with poor prognosis was significantly higher than that in the control group (P＜0.05), but there were no differences in age, gender and degree of differentiation between the two groups (P>0.05). Multivariate Logistic regression analysis with clinical stage, cervical lymph node metastasis, PAC-1, CD62P and SLC7A11 as independent variables showed that clinical stage, cervical lymph node metastasis, PAC-1, CD62P and SLC7A11 were independent risk for recurrence (P＜0.001). Conclusion PAC-1, CD62P and SLC7A11 are highly expressed in elderly patients with laryngeal squamous cell carcinoma, and are closely related to the prognosis of patients, which may be used as a reference indicator for prognosis evaluation.

Key words: The elderly laryngeal squamous cell carcinoma, PAC-1, CD62P, SLC7A11, Prognosis, Risk factors