Abstract:

Objective To discuss the platinum related excision repair cross-complementation group 1（ERCC1） mRNA expression in different histological types of lung cancer and its corresponding blood cells， and to explore the clinical significance. Methods Real-time quantitative PCR was used to detect ERCC1 mRNA expression of 105 lung cancer cases（73 adenocarcinoma， 22 squamous cell carcinoma and 10 small cell carcinoma） from paraffin-embedded tissues and control blood samples， and the different expressions of ERCC1 mRNA in different tissue types were analyzed. ResultsERCC1 mRNA expressions were 4.579±1.926 and 8.324±1.280 in 105 paraffinembedded tissues and the corresponding blood samples, showing significant differences（P＜0.05）. In 105 lung cancer specimens，ERCC1 mRNA expressions in lung squamous cell carcinoma， adenocarcinoma and small cell carcinoma from paraffinembedded tumor cells were 4.366±1.822， 4.724±1.434 and 4.366±1.822， with no difference among the three histological types of lung cancer. The expressions in blood cells were 8.146±1.501，8.336±1.928 and 8.664±1.848， without significance among the three types. ERCC1 mRNA expression was significant differences between paraffinembedded tumor cells and corresponding blood cells in all the three tissue types（P＜0.05）. The expressions had no correlation in tumor cells and blood cells（r=0.329， P＞0.05）. Conclusion Histological types of lung cancer cannot guide platinum based chemotherapy， and further individual ERCC1 mRNA detection is needed. Using blood instead of the tumor tissues in individual detection of ERCC1 gene is not advised.