Abstract: Objective To investigate CD133 expression in nonsmall cell lung cancer（NSCLC） and the relationship with prognosis. Methods Such databases as PubMed， CBMdisc， Cochrance， CNKI， Wanfang and Weipu database were searched from the date of their establishment to March 15th 2013 to collect the researches about CD133 expression and prognostic significance in NSCLC. According to the inclusion and exclusion criteria， two reviewers screened literature， extracted data and assessed the quality of included studies independently. Then results of metaanalysis were conducted using RevMan 5.0 software. Results A total of 11 researches involving 947 patients were included. The results of metaanalysis suggested that CD133positive rate was correlated to nodal metastasis（RR=1.33, 95%CI:1.17-1.51, P＜0.0001）， and CD133positive patients had low 3-and 5-year overall survival compared with CD133-negative patients（P＜0.05）. But CD133-positive rate was not related to gender， smoking history， pathological types， histology grading and T stage. Conclusion NSCLC patients with high expression of CD133 may have lower overall survival. The quality and quantity limitation of the included studies decrease the strength of evidence， so the conclusion of this systematic review only provides some references for clinical research and practice.