Abstract: Objective To analyze the mutation status of v-raf murine sarcoma viral oncogene homolog B1（BRAF） gene in Chinese colorectal cancer（CRC）patients，and to promote antiepidemal growth factor receptor（EGFR）therapy. Methods The mutation of BRAF gene in 169 CRC tissues were detected by PCR direct sequencing. Results The mutation was found at the 600th codon（V600E）of BRAF gene, and the mutation frequency was 5.3％ in 169 CRC tissues. Moreover，the mutation frequency of BRAF gene was 7.1％ in 84 male and 3.5％ in 85 female CRC without statistical significance（P=0.329）. No mutation was found in 17 CRC youth patients（25-44 years old），the mutation frequency was 3.0％ in 67 CRC middle age patients（45-59 years old），and 8.2％ in 85 CRC elder patients（60-90 years old）without statistical significance（P=0.211）. Conclusion The mutation frequency of BRAF gene in CRC patients is not related to gender and age.