Abstract: Objective To investigate the level of miR-455-3p in gastric carcinoma and its relationship with microvascular density （MVD） and vascular endothelial growth factor （VEGF）. Methods The quantitative real-time PCR was used to examine the level of miR-455-3p in 80 gastric carcinoma tissues （gastric carcinoma group）. The vascular endothelial growth factor （VEGF） expression and microvascular density （MVD） were measured by immunohistochemical method in 80 gastric carcinoma tissues. The correlations between level of miR-455-3p and MVD， VEGF expression were analyzed. Thirty-seven superficial gastritis and 12 normal gastric mucosa tissues were chosen as control （control group）. Results The level of miR-455-3p in gastric carcinoma group was 1.16±0.59， lower than 2.61±0.88 in control group with significant difference （P＜0.05）. The gastric carcinoma group’s positive expression rates of VEGF and MVD were 71.3％ （57／80） and 54.9±7.3， higher than 28.6％ （14／49） and 27.5±6.1 in control group with significant difference （P＜0.05）. The miR-455-3p was negatively correlated with VEGF expression （r=-0.783， P＜0.05） and MVD （r=-0.824， P＜0.05） in gastric carcinoma. Conclusion There was a decreased level of miR-455-3p in gastric carcinoma. The miR-455-3p was negatively correlated with VEGF expression and MVD in gastric carcinoma， which may play an important role in the regulation of blood vessel growth.