Abstract: Objective To investigate the effect of donkeyhide gelatin mixture on myelosuppression induced by gemcitabine and cisplatin （GP） chemotherapy. Methods According to the treatment， 119 patients with malignant tumor from 2011 January to 2012 June were assigned into treatment group（n=64） and control group（n=55）. Both groups received GP regime （gemcitabine， 1g／m²， d₁， d₈； cisplatin， 20mg／m²， d₁-d₈； 21 days was a cycle） for chemotherapy. Only the treatment group received donkeyhide gelatin mixture （20ml per time， tid） from 1-2 days before chemotherapy to one week after the end of chemotherapy. The usage of chemotherapy of interleukin11 （rhIL-11） and recombinant human granulocyte colony stimulating factor （rhG-CSF） during chemotherapy were compared between two groups. The white cell counts and platelet counts before the 1st and 2nd cycle of chemotherapy， minimum values of white cell count and platelet count after 1st and 2nd cycle of chemotherapy and postponed rates caused by bone marrow suppression were recorded between groups. The Response Evaluation Criteria in Solid Tumors （RECIST） criteria （version 1.1） was employed to evaluate the shortterm effect after chemotherapy for 2 cycles.
Results The usage of both rhIL-11 and rhG-CSF in treatment group were lower than those in control group （P＜0.05）. No statistically significant difference was observed between both groups on platelet counts and blood cell counts before the 1st and 2nd cycle of chemotherapy （P＞0.05）. The minimum values of platelet count were higher in treatment group versus control group after the 1st and 2nd cycle of chemotherapy （P＜0.05）， but the minimum values of blood cell counts were similar between groups （P＞0.05）. The treatment group completed 107 cycles of chemotherapy on schedule， while the control group completed 75 cycles of chemotherapy on schedule. The postponed rates of treatment group was lower than that of control group （16.2％ vs. 32.1％， P＜0.05）. The response rates and disease control rates were 49.09％ and 85.45％ in control group and 43.75％ and 79.69％ in treatment group without significant differences （P＞0.05）. During the process of donkeyhide gelatin mixture， no clear discomfort and side effects were observed. Conclusion Donkey-hide gelatin mixture can relieve the myelosuppression caused by GP regimen chemotherapy and increases blood platelets count after chemotherapy. The longterm effects on chemotherapy efficacy needed to be observed， and the mechanism for the efficacy should be investigated furthermore.