Abstract: Objective To estimate the effects of a novel radiofrequency （RF） ablation catheter， the HabibTM EndoHPB， with various settings of powers and times. Methods We used resected fresh pig livers to examine at 37.0 ℃. The RF application was performed step by step at 5， 10， and 15 W power and 60， 90， 120，150s， respectively. The change of temperature， macroscopic（the lengths of the short and long axes） and microscopic findings of the ablation area were evaluated at each setting. Results The highest temperature of the ablation area at 5W power and 60， 90，120 and 150s were 42.0℃ to 54.0℃， at 10W power and 60， 90， 120 and 150s were 52.0℃ to 70.0℃， and at 15W power and 60， 90 and 120s were 62.0℃ to 77.0℃. The lengths of the short axis of the ablation area at 10W power and 60， 90， 120 and 150s were （8.5±0.8）mm， （9.0±0.8）mm， （9.5±0,8）mm and （10,0±0,8）mm， respectively. The lengths of the long axis at 10W power and 60， 90，120 and 150s were （21.0±1.0）mm， （23.5±1.0）mm， （25.5±1.0）mm and （30.0±1.0）mm， respectively. Although the lengths of the short and long axes at 5， 10 and 15W increased gradually with power， there were no obvious differences in either short or long axis lengths between 10 and 15W power（P＜0.05）. Microscopic examination of the ablationareashowed liver tissue dissociationed， cell spacing increased and extensive cellular degenerationed. The degree increased as power／time increased. And liquefactive necrosis sheet was appeared at 10W power and 120s or 15W power and 150s. Conclusion Although the clinical ablation setting at 10W power and 90 to 120s is suitable， ultimately the ablation power and time should be adjusted according to our experimental result.