Abstract:

Objective To investigate the relationship between the mutations of K-Ras and epidermal growth factor receptor（EGFR） gene and K-Ras gene and clinicopathological features in Chinese patients with non-small cell lung cancer（NSCLC）. Methods From July 2011 to August 2013， 381 cases of NSCLC patients with both EGFR and K-Ras mutations tested in the First Affiliated Hospital of Guangzhou Medical University were retrospectively enrolled in the study. All pathological specimens were tested for twenty-one mutations in EGFR 18-21 exon and six mutations in K-Ras 12，13 codon by amplification refractory mutation system（ARMS）. The clinicopathological features of patients were analyzed according to the mutation status of EGFR and K-Ras. Results Gene mutation of K-Ras was tested in 21 cases， including 20 cases 12 codon mutation and 1 case 13 codon mutation. Gene mutation of EGFR was tested in 146 cases， including 4 cases of 18 exon G719S mutation， 52 cases of 19 exon delection mutation， 3 cases of 20 exon delection mutation， 85 cases of 21 exon mutation （81 cases of L858R and 4 case of L861Q） and two dual gene mutation. K-Ras mutation was more frequently happened in male patients than in female patients （6.8％ vs. 2.5％， P=0.018）. EGFR mutations were related with gender, smoking history, TNM stage, systemic metastases and pathological type（P＜0.05）. Binary Logistic regression shown that pathological type and gender were closely related to EGFR mutation（P＜0.05）. Conclusion EGFR mutation was common in Chinese patients with NSCLC， and was related to adenocarcinoma. However， K-Ras mutation was rare and more commonly happened in male patients with NSCLC. More studies should be conducted to investigate the relationship between clinical features and EGFR and K-Ras mutation.