Chinese Clinical Oncology

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Influence of nm23-H1 gene transfection on expressions of MMP-9 and TIMP-1 in lung cancer cells

ZHENG Haixia, CUI Yongyan, SHEN Donglan, PENG An, HE Yanling.   

  1. Department of Health Examination, Peking University Shenzhen Hospital
  • Received:2014-08-25 Revised:2015-11-09 Online:2015-02-28 Published:2015-02-28
  • Contact: CUI Yongyan

Abstract: Objective To investigate the influence of nm23-H1 gene transfection on the expressions of matrix metalloproterinase 9(MMP-9)and tissue inhibitor of metalloproteinase 1(TIMP-1) in lung cancer cells. Methods nm23-H1 gene was transfected into lung cancer cell line L9981 by gene transfection technology. SemiRT-PCR was used to detect the different mRNA expressions of TIMP-1 and MMP-9 between transfected and nontransfected lung cancer cell lines. Flow cytometry was used to detect the different protein expressions of TIMP-1 and MMP-9 between transfected and non-transfected lung cancer cell lines. Results The mRNA and protein levels of TIMP-1 in transfected lung cancer cell lines were stronger than those in non-transfected lung cancer cell lines. The mRNA and protein levels of MMP9 in transfected lung cancer cell lines were weaker than those in non-transfected lung cancer cell lines. Conclusion The nm23-H1 gene can up-regulate expressions of TIMP-1 and down-regulate expressions of MMP-9 in lung cancer cell line L9981.

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