Abstract: Objective To explore the effects of bromodomain-containing protein 4 （BRD4） inhibitor（GSK525762A） on proliferation， apoptosis and invasion of nasopharyngeal carcinoma cell line CNE-2. Methods The nasopharyngeal carcinoma cell line CNE-2 was treated by GSK525762A （0， 0.1， 1， 10， 100 μmol／L） for 24， 48， 72 and 96 h. The methyl thiazolyl tetrazolium salt （MTT） was used to detect the proliferation inhibition rates at the above time points. Meanwhile， the apoptotic rates of CNE-2 cells at 48， 96 h after treatment with GSK525762A were measured with Annexin-FITC／PI double staining via flow cytometry. Transwell method was used to detect the invasion ability of CNE-2 cells at 48， 96 h after treatment with GSK525762A. The realtime quantitative PCR was performed to detect expressions of apoptosis related genes at 48， 96 h after treatment with GSK525762A. Results There was inhibitory effect of GSK525762A on the proliferation of CNE-2 cells， and the inhibition rate was increased in a time- and concentration-dependent manner （P＜0.05）. After the treatment of GSK525762A， the early， late and total apoptotic rates increased with the increase of concentrations， higher than those of 0 mol／L group （P＜0.05）； the number of transmembrane cells of other concentrations were lower than that of 0 mol／L group （P＜0.05）； Compared with the 0 μmol／L， there were lower level of mRNA in Bcl-2 but higher levels of mRNA in Bax and Bak with the range （0.1-100 μmol／L） （P＜0.05）. Conclusion BRD4 inhibitor GSK525762A presented toxicity on the proliferation of CNE-2 cell， induced apoptosis and restored the expressions of apoptosis related genes.