Abstract:

Objective To investigate the inhibitory effects of toxoflavin on proliferation， apoptosis and migration of non-small cell lung cancer A549 cells. Methods The cultured A549 cells were treated with toxoflavin（0.5， 0.375， 0.25， 0.125 μmol／L） for 24 h and 48 h， the control group was treated with cell culture media only. CCK-8 assay was used to test the growth inhibitory rate and Annexin V／propidium iodide（PI）staining assay was applied to detect the apoptosis rate. The cell migration rate of 12 h， 24 h， 48 h were measured by the wound healing assay. Results The cell growth inhibition rates of A549 cells in experimental groups were （93.51±3.69）％，（40.38±3.08）％，（23.54±2.58）％，（13.07±2.37）％ in 24 h and（90.53±3.58）％，（53.72±3.02）％，（34.44±3.10）％，（24.78±2.43）％ in 48 h. The cell apoptosis rates of A549 cells in expremental groups were （78.68±2.22）％，（43.66±2.53）％，（20.81±2.59）％，（6.25±0.96）％ in 24 h and（88.66±3.16）％, （59.86±2.81）％，（27.89±3.48）％，（9.91±1.33）％ in 48 h， and those of control group were（1.57±0.52）％ in 24 h and（1.59±0.55）％ in 48 h respectively. The A549 cell migration rate of 12 h，24 h，48 h in experimental group（0.125 μmol/L） was 7％，11％ and 16％，but it was 14％，26％ and 39％ in control group. Conclusion Toxoflavin could significantly inhibit proliferation and promote apoptosis of A549 cells in a dose and time dependent manner， and it could also weaken the migration capacity of A549 cells.