Abstract: Objective To investigate the value of conventional MRI and proton magnetic resonancespectroscopy（1H-MRS）in the diagnosis and differential diagnosis of high-grade gliomas and solitary brain metastasis. Methods Twenty-seven patients with high-grade gliomas and 21 patients with solitary brain metastasis were confirmed by surgery and pathology in our hospital. Conventional MRI and 1H-MRS examination were performed. The imaging features and the changes of 1HMRS metabolites， including N-acetylaspartate（NAA）， choline（Cho） and creatine（Cr） were analyzed. Results There were differences between high-grade gliomas and solitary metastasis in location and edema extent of the lesions in conventional MRI. As for 1H-MRS， it has statistical significance between high-grade gliomas and solitary brain metastasis body region in Cho／NAA value（P＜0.05）， but no statistical significance was observed between NAA／Cr and Cho／Cr values（P＞0.05）. There were statistically significance in peri-tumor region in Cho／Cr and Cho／NAA values（P＜0.05）, while NAA／Cr value has no statistically significance（P＞0.05）. Conclusion The accuracy of conventional MRI and 1H-MRS in diagnosis and differential diagnosis of high-grade gliomas and solitary brain metastases is further improved， and it has high clinical application value.