Abstract: Objective To examine the expression of Dishevelled-1 （Dvl1）， Cyclin D1 and CD44v6 in pediatric osteosarcoma tissues， and investigate the correlations among them and the clinicopathological factors. Methods The expressions of Dvl1， Cyclin D1 and CD44v6 were detected in 47 pediatric osteosarcoma tissues by immonohistochemistry method. The relationship among Dvl1， Cyclin D1， CD44v6 and clinicopathological factors was investigated. Results The high expression rates of Dvl1，Cyclin D1 and CD44v6 were 76.60％ （36／47）， 72.34％ （34／47） and 68.09％ （32／47）， respectively in pediatric osteosarcomas. The expression of Dvl1 was correlated with the tumor diameter （P=0.020） and Ennecking stage （P=0.032）. Similarly， the expression of Cyclin D1 and Cd44v6 were also correlated with the tumor diameter （P=0.007 and P=0.036） and Ennecking stage （P=0.024 and P=0.020） of pediatric osteosarcomas. Moreover， the expression of Dvl1 was positively correlated with Cyclin D1 expression （r=0.332，P=0.023） and CD44v6 expression （r=0.376，P=0.009）， respectively. Conclusion Dvl1， Cyclin D1 and CD44v6 were highly expressed in pediatric osteosarcoma. The high expressions of Dvl1， Cyclin D1 and CD44v6 can promote the malignant progression of pediatric osteosarcomas.