Abstract:

Objective To explore the effect of small interfering RNA（siRNA）targeting protein arginine methyltransferase 5（PRMT5）on the proliferation and colony-formation in human gastric cancer SGC7901 cells. Methods siRNA-1 and siRNA-2 designed for specific targeting PRMT5 were transiently transfected into SGC7901 cells. Meanwhile，antisense sequence was used as negative control（NC）. 48 h after transfection，Western blotting method was used to confirm the interfering efficacy to ensure that siRNA can be used for following experiments. CCK-8 assay was used to detect the proliferation of SGC7901 cells after gene-specific siRNA was transfected. EdU assay was used to examine the DNA replication of SGC7901 cells and plate colonyformation assay was used to evaluate the colony-formation of SGC7901 cells when PRMT5 was silenced by siRNA. Results The level of PRMT5 protein was significantly decreased in SGC7901 cells when siRNA-1 and siRNA-2 were transfected，suggesting that siRNA-1 and siRNA-2 could be used for further studies. The proliferation rate of SGC7901 cells was significantly reduced when PRMT5 was silenced（P＜0.01）in comparison with NC. Compared with NC，the positive rates of EdU cells interfered by siRNA-1 and siRNA-2 in PRMT5 group were（16.0±2.0）％ and（19.5±3.0）％，much lower than（38.0±4.0）％ in NC with significance（P＜0.01）. Furthermore，the numbers of SGC7901 colonies in PRMT5 group interfered by siRNA-1 and siRNA-2 were 50±6 and 68±7，lower than 120±11 of NC with significance（P＜0.01）. Conclusion Knockdown of PRMT5 by gene-specific siRNA can inhibit the proliferation and colony-formation abilities in human gastric cancer SGC7901 cells，which provide new methods and theoretical basis for the treatment of gastric cancer.