Abstract: Objective To explore the expression of long non-coding RNA of colon cancer associated transcript-2（LncRNA CCAT-2） in hepatocellular carcinoma（HCC） tissues as well as its clinical significance. Methods The expression level of CCAT-2 in 60 pairs of HCC tissues which were pathologically confirmed as HCC postoperatively and paired adjacent normal tissues were tested by real-time PCR， and then we investigated the relationship between the expression of CCAT-2 with clinical pathological features such as gender， age， preoperative alpha-fetoprotein（AFP） level， HBsAg， tumor size， number of tumors， tumor stage， portal vein tumor thrombus（PVTT）and differentiation grade. Results The expression of CCAT-2 in HCC tissues elevated significantly with an average fold of 5.26±2.08.The difference was statistically significant（P=0.001）. The level of CCAT-2 expression was not associated with the gender， age， HBsAg and number of tumors（P>0.05）， but significantly related with the AFP level， tumor size， tumor stage，PVTT and differentiation degree（P<0.05）. Conclusion CCAT-2 may play a promoting role in the development， invasion and migration process of HCC.