Abstract: Objective To investigate the expression and significance of DAB2IP in colorectal carcinoma in mRNA and protein levels. Methods One hundred and twentyseven cases of colorectal carcinoma tissues and 36 cases of adjacent normal colorectal tissues were examined by immunohistochemistry and nucleic acid in situ hybridization. The relationship between the expression of DAB2IP mRNA and protein with clinicopathological characteristics such as lymph node metastasis， differentiation grades and Dukes stages in colorectal carcinoma. Results The positive expression rate of DAB2IP mRNA and protein in colorectal carcinoma tissues was 85.04％，79.53％， lower than 100.00％， 94.45％ in adjacent normal tissues with significant difference （all P＜0.001）. DAB2IP was gradual highexpression in high， medium and low differentiative groups， and there was significant difference in three groups （P＜0.01）. The positive expression rate of DAB2IP in non-lymph node metastasis group was significantly higher than that in lymph node metastasis group， and there were negatively related （r=-0.2361， r=-0.3060； P＜0.01）. DAB2IP was gradual downexpression from Dukes A to Dukes D stages （P＜0.01）. The expression of DAB2IP was not related to age， sex， tumor site， and tumor volume of colorectal carcinoma（P＞0.05）. Conclusion DAB2IP has significant effect in the occurrence and development of colotretal carcinoma. The downexpression of DAB2IP is closely related to metastasis in colorectal carcinoma， which is a potentially important indication for the biological behaviors and prognosis of colorectal carcinoma.