Abstract:
【Abstract】Objective   To investigate the expression of lncRNA SNHG5 in hepatocellular carcinoma（HCC） tissues， and explore its biological regulation on cell proliferation and cell apoptosis. Methods   The mRNA expression level of SNHG5 in hepatocellular carcinoma tissues and adjacent normal tissues was determined by QPCR. Then HepG2 cells were transfected with siRNA to knockdown the expression of SNHG5. CCK-8， flow cytometry， QPCR and Western blotting were used to detect the biological characteristics of proliferation and apoptosis of HepG2 cells， as well as the changes in the expression level of apoptosis pathway related markers. Results  The expression level of SNHG5 in HCC tissues was significantly higher than that in matched adjacent tissues （P＜0.05）. After knocking down SNHG5 expression， the proliferation and anti-apoptotic ability of HepG2 cells were inhibited， and the expressions of apoptotic proteins cleaved caspase-3 and BAX were upregulated. Conclusion   SNHG5 was found to be overexpression in liver cancer tissues. SNHG5 might play as a potential therapeutic targetby regulating proliferation and apoptosis of HCC.

Key words: Hepatocellular carcinoma, SNHG5, Proliferation, Apoptosis, Caspase-3