Abstract: Objective To investigate the relationship between the expression of PI3K， Akt and Survivin and clinicopathological features and prognosis in gastric cancer， and to provide theoretical basis for the diagnosis and prognosis evaluation of gastric cancer. Methods  The expression levels of PI3K， Akt and Survivin were detected in 75 gastric cancer tissues using En Vision immunohistochemical method.The relationship between the expression and the clinicopathological features was analyzed. In addition， Spearman correlation test was used to analyze the relationship between the expressions of PI3K， Akt and Survivin. Survival analysis was performed by Kaplan-Meier method， and Cox multivariate regression analysis was used to identify the independent prognostic factors of gastric cancer. Results  In gastric cancer tissues， the positive rates of PI3K， Akt and Survivin were 73.3 ％（55／75）， 38.7％ （29／75） and 61.3 ％（46／75） respectively. The expression of PI3K， Akt and Survivin was related to the degree of differentiation， TNM staging and lymph node metastasis （P＜0.05）， but not with sex， age， size of the mass， and the level of CEA before treatment （P＞0.05）. The expression of PI3K was positively correlated with Akt and Survivin expression （r=0.293， P＜0.05； r=0.450， P＜0.05）. PI3K， Akt，  Survivin， differentiation， TNM staging and lymph node metastasis were correlated with prognosis in gastric cancer. Cox regression analysis showed that Survivin， lymph node metastasis were independent prognostic factors of gastric cancer （P＜0.05）. Conclusion  The expression of PI3K， Akt and Survivin is closely related to the invasion and metastasis of gastric cancer. There may be a synergistic effect between PI3K and Akt， PI3K and Survivin， which jointly promote the malignant progression of gastric cancer. Survivin is one of the independent factors that affect the prognosis of gastric cancer.

Key words: Gastric cancer, PI3K, Akt, Survivin, Immunohistochemistry, Prognosis