Abstract:

Objective  To investigate the expression and significance of miRNA-138-5p （miR-138-5p） in non-small cell lung cancer （NSCLC）. Methods Real-time fluorescence quantitative PCR （QPCR） was used to detect the expression of miR-138-5p in 102 NSCLC tissues and 98 paracancerous tissues. The relationship between the expression of miR-138-5p and the clinicopathological features of NSCLC （sex， age， TNM stage， tumor size and lymph node metastasis） were analyzed. The survival curve was drawn by Kaplan-Miere method， and the difference was checked by Log-rank test. Cox regression model was used to analyze the factors affecting overall survival（OS）. Results The expression level of miR-138-5p in NSCLC tissues was 0.42±0.11， significantly lower than 1.03 ±0.28 in adjacent tissues （P＜0.05）. The expression of miR-138-5p was correlated with TNM stage， tumor size and lymph node metastasis （P＜0.05）. The median OS of high expression of miR-138-5p was more than 36 months， which was significantly higher than that of low expression of miR-138-5p （P＜0.05）. Cox univariate analysis showed that besides the expression of miR-138-5p， lymph node metastasis， tumor size and TNM stage were also associated with OS （P＜0.05）. Conclusion The expression of miR-138-5p is down-regulated in NSCLC， which is related to the development and prognosis of NSCLC. MiR-138-5p can be used as a new target for diagnosis and prognosis prediction of NSCLC.

Key words: Non-small cell lung cancer（NSCLC）, miRNA-138-5p, Prognosis