Abstract:

ObjectiveTo explore the correlation between singlenucleotide polymorphisms（SNPs） of telomerase reverse transcriptase（TERT） rs2736098 and rs2736100 and the susceptibility to gastric cancer as well as the infection of Helicobacter pylori（Hp）. Methods This casecontrol study included 297 diagnosed gastric cancer patients and 306 nonchronic atrophic gastritis patients. Polymerase chain restriction fragment length polymorphism and direct sequencing were performed to analyze the genotype of rs2736098 and rs2736100 on TERT. Hp infection was detected by pathological diagnosis and 13Curea breath test. ResultsThe frequencies distribution of three genotypes of rs2736098 had no significant difference between gastric cancer and control group（P＞0.05）. The frequency of GG genotype of rs2736100 was higher in the gastric cancer group compared to the control group（27.0％ vs. 16.4％， P＜0.05）. The multivariates Logistic regression analysis showed that GG genotype carriers had an increased risk of gastric cancer compared with the TT carriers（OR=1.371， 95％CI：1.063-1.775， P=0.005）. In negativeHp infection patients， GG genotype carriers had higher risk of gastric cancer than TT genotype carriers（OR=1.421, 95%CI:0.988~2.042,P=0.046）. There was no assoliation between Hp infection and genetic variation. Conclusion The polymorphism of rs2736100 on TERT may play an important role in susceptibility to gastric cancer, but not relate to Hp.