Abstract: Objective To investigate the ANXA1 expression in metastasis carcinoma of lymph node and determine its clinical significance in gastric cancer. Methods Tissue microarray blocks containing tumor specimens obtained from 121 patients with gastric cancer were constructed. Expressions of ANXA1 and p-mTOR in these specimens were analyzed by immunohistochemical method. Results The expression of ANXA1 was observed in 18 cases（14.9％）of 121 metastasis tumors and the expression of p-mTOR was observed in 74 cases（61.2％）of 121 metastasis tumors by immunohistochemistry method, while their expression in primary tumor location was 35.5% and 51.2%. The loss of ANXA1 expression was more edident than its expression in primary tumors and its loss expression was significantly associated with primary tumor location，T stage, expression of p-mTOR，and histological differentiation. The loss of ANXA1 expression had a significant correlation with poor survival in univariate analyses. Conclusion The loss of ANXA1 expression plays a very important role in the metastasis of lymph node. ANXA1 expression may be regarded as a biomark to predict prognosis.