不同肺癌细胞株中VEGFR-2 mRNA表达的实验研究

临床肿瘤学杂志

不同肺癌细胞株中VEGFR-2 mRNA表达的实验研究

张莹1,2，蒋晓东3，乔云3，刘毅3，刘亮3，丁曼华3

1 221006 徐州徐州医学院研究生院 2 222042 解放军第149医院消化肿瘤科 3 222002 连云港市第一人民医院肿瘤科

收稿日期:2013-06-05 修回日期:2013-07-20 出版日期:2013-10-31 发布日期:2013-10-31
通讯作者: 蒋晓东

The expressions of VEGFR2 mRNA in different lung cancer cell lines

ZHANG Ying， JIANG Xiaodong， QIAO Yun， LIU Yi， LIU Liang， DING Manhua

Graduate School, Xuzhou Medical College, Xuzhou 221006，China

Received:2013-06-05 Revised:2013-07-20 Online:2013-10-31 Published:2013-10-31
Contact: JIANG Xiaodong

摘要/Abstract

摘要： 目的研究不同非小细胞肺癌（NSCLC）细胞株中血管内皮生长因子受体-2（VEGFR-2） mRNA的表达情况。方法采用实时荧光定量PCR法检测人肺黏液表皮样癌NCI-H292细胞株、人肺腺癌NCI-H1299细胞株、人肺腺癌A549细胞株、人肺巨细胞癌95D细胞株和人肺鳞癌Calu-1细胞株中VEGFR-2 mRNA的表达。结果 Calu-1、NCI-H292、95D、NCI-H1299和A549细胞株中VEGFR-2 mRNA表达依次为1.213±0.134、1.008±0.164、0.754±0.088、0.582±0.171和0.121±0.026，差异有统计学意义（F=31.875， P=0.000）。Calu-1与NCI-H292细胞株间VEGFR-2 mRNA表达无统计学差异（P=0.080），NCI-H1299与95D细胞株间VEGFR-2 mRNA表达亦无统计学差异（P=0.137），其余细胞株间差异均有统计学意义（P均＜0.05）。结论不同NSCLC细胞株中VEGFR2 mRNA表达量不同，可能与不同肿瘤细胞的生物学行为有关。

Abstract: Objective To investigate the expressions of vascular endothelial growth factor receptor 2 （VEGFR-2） mRNA in different lung cancer cell lines. Methods The expressions of VEGFR2 mRNA in the human pulmonary mucoepidermoid carcinoma cell line NCI-H292， lung adenocarcinoma cell line NCI-H1299， lung adenocarcinoma cell line A549， lung giant cell carcinoma 95D and lung squamous carcinoma cell line Calu-1 were detected by real-time quantitative PCR （RT-PCR）. Results The expressions of VEGFR-2 mRNA were 1.213±0.134， 1.008±0.164，0.754±0.088， 0.582±0.171 and 0.121±0.026 in Calu-1，NCI-H292，95D，NCI-H1299 and A549 cell lines， showing significant differences （F=31.875， P=0.000）. No significant difference was observed in the expression of VEGFR-2 mRNA between Calu-1 and NCI-H292 （P=0.080） or between NCI-H1299 and 95D （P=0.137）. Conclusion The expression of VEGFR-2 mRNA is diverse in different lung cancer cell lines， which is closed with the different biological behavior.

张莹1,2，蒋晓东3，乔云3，刘毅3，刘亮3，丁曼华3. 不同肺癌细胞株中VEGFR-2 mRNA表达的实验研究[J]. 临床肿瘤学杂志, 2013, 18(10): 879-.

ZHANG Ying， JIANG Xiaodong， QIAO Yun， LIU Yi， LIU Liang， DING Manhua . The expressions of VEGFR2 mRNA in different lung cancer cell lines [J]. Chinese Clinical Oncology, 2013, 18(10): 879-.

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