Abstract: Objective To investigate the influence of californium-252（252Cf） neutron ray on the expressions of the glioma-associated oncogene homolog 1（Gli-1） and vascular endothelial growth factor（VEGF） in tumor tissues from cervical cancer patients.Methods The cervical cancer tissues from 25 cervical cancer patients receiving 252Cf neutron ray brachytherapy and external irradiation were collected. The expressions of VEGF and Gli-1 before and after radiotherapy in cervical cancer tissues were detected by immunohistochemical SP method and western blot，respectively. The VEGF positive cells and the Gli-1 relative optical density of different pathological parameters before and after radiotherapy were compared. Five cases of cervicitis tissues were chosen as control. Results The high expression rate of VEGF and the relative optical density of Gli-1 in cervical cancer tissues before radiotherapy were 80.0％ and 0.80±1.73，higher than 44.0％ and 0.41±0.65 after radiotherapy and 20.0％ and 0.20±0.50 in cervicitis tissues with significant differences（P＜0.05）. The VEGF positive cells and the Gli-1 relative optical density after treatment of different stages and differentiation levels were lower than before（P＜0.05）.
ConclusionThe 252Cf neutron ray can significantly decrease the expressions of VEGF and Gli-1.