Abstract: Objective To investigate the expression of Toll-like receptor-2（TLR-2）and Toll-like receptor-5（TLR-5）in diffuse large B-cell lymphoma（DLBCL）, as well as the relationship of their expression with clinical features. Methods Immunohistochemistry stain was used to determine the expression of TLR-2 and TLR-5 in 60 DLBCL tissues. Twenty-one lymph node reactive hyperplasia tissues were taken as control. The relationship between TLR-2，TLR-5 expression and clinical features was analyzed. Results Positive rates of TLR-2 in DLBCL tissues and control group were 78.3％ and 28.5％（P＜0.05）. The expression of TLR-2 was related with clinical stage，LDH，IPI，subtypes，out of lymph node invasion and systemic symptoms，but not related to age, gender and ECOG score. Positive rates of TLR-5 in DLBCL tissues and control group were 26.6％ and 23.8％（P＞0.05）, and there was no relationship between it and clinical features. Conclusion TLR-5 has no relationship with clinical features of DLBCL；TLR-2 may be related to the occurence and prognosis of DLBCL，could be regarded as an biomarker of prognosis of this disease.