Abstract: Objective To observe the curative effect of cinobufacini injection infusion therapy in advanced lung cancer with malignant pericardial effusion. Methods Six advanced lung cancer patients with malignant pericardial effusion were collected by echocardiography. The cinobufacini injection was perfused into pericardial cavity at a dose of 6-10ml twice a week with a twoweek cycle after draining all of the pericardial effusion. Meanwhile， the symptomatic treatments （rehydration， diuresis， electrolyte balance， etc.） were given. The objective response was evaluated by effusion in serous cavities evaluation criteria established by WHO and response assessment in RECIST version 1.1. The adverse reactions were evaluated by NCI CTC 3.0. Results The effusion was dark red， concentrated and bloody with average drainage flow of 800 ml each time. The pericardial effusion was under control after 24 times of pericardial cavity injections with 4 times for each patient at average. There were 4 cases of complete remission and 2 cases of partial remission. In addition to one patient with mild gastrointestinal reactions，there was no blood toxicity （leucopenia， thrombocytopenia， decreased hemoglobin level， etc.）. Conclusion Cinobufacini injection infusion therapy of advanced lung cancer with malignant pericardial effusion could control the pericardial effusion， improve the patient's quality of life and prolong survival to a certain degree with few side effects. It is worthy of further clinical observational study.